期刊论文

Yoon, Juyoung

英文

Nature Protocols

1754-2189

2015

10

11

1742-1754

10.1038/nprot.2015.109

acknoWleDGMents This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (grant no. 2012R1A3A2048814), by the Basic Science Research Program through the NRF funded by the Ministry of Education (grant no. 2013R1A1A2008511), and by the Yonsei University College of Medicine (grant no. 6-2014-0135 to J.-H.R.) and the National Natural Science Foundation of China (grant no. 21402057).

本校为其他机构

Glutathione (GSH) is a major endogenous antioxidant that has a central role in cellular defense against toxins and free radicals. This protocol describes the preparation of CPDSA, a cyanine-based near-infrared (NIR) fluorescent probe for the detection of GSH in cells and in vivo. CPDSA is prepared with high yield through a simple two-step process. The first step is to react commercially available IR-780 iodide with excess anhydrous piperazine in anhydrous N,N-dimethyl formamide at 85 °C to form cyanine-piperazine (CP). The second step is the s...