Large amounts of trifluralin are applied each year for weed control;however, its effects on soil microbial communities and functions are unknown. Two agricultural soils, one silty loam and one silty clay were spiked with TFL (0, 0.84, 8.4, and 84 mg kg–1) and studied the effects using a laboratory microcosm approach. The half-lives were 44.19–61.83 d in all cases. Bacterial abundance increased 1.12–5.56 times by TFL, but the diversity decreased. From the next-generation sequencing results, TFL altered the bacterial community structure, which initially diverged from the ...