Cytochrome P-450nor is involved in the fungal denitrification and acts as a nitric oxide reductase. Tha cDNA library from Cylindrobarpon tonkinense was constructed with λgtll, and screened with antibodies. From the positive clones, the P-400nor2 cDNA fragments were recovered, and subcloned into the expression vector pYES2, then expressed in the yeast system. Western-blot analysis showed that the expressed protein, was hybridized with the antibody. Enzyme assay indicated that the expressed protein had the activities of P-450nor2, which reduc...
