期刊论文

中文

化学与生物工程

1672-5425

2005

22

6

4-5,20

10.3969/j.issn.1672-5425.2005.06.002

国家重点基础研究发展规划项目（2003CB114400）； 国家自然科学基金资助项目（20072008,20372023）；

本校为第一机构

以不同浓度(10-3～10-7g·mL-1)的O-甲基α-(2,4-二氯苯氧基乙酰氧基)甲基膦酸酯单钠盐(以下简称WT)分别在体外处理水稻幼芽和从水稻线粒体中提取的丙酮酸脱氢酶复合体,结果显示随着WT浓度的逐渐降低,其细胞相对存活率逐渐升高;随着WT浓度的逐渐增高,对丙酮酸脱氢酶的抑制活性也逐渐增强.WT对丙酮酸脱氢酶的抑制活性的I50为29.14 μmol·L-1.由此说明化合物WT是丙酮酸脱氢酶的强抑制剂.

Effects of O-methyl α-( 2,4-dichlorophenoxyacetoxy) methylphosphonate monosodium (WT) on rice budlet and pyruvate dehydrogenase from rice mitochondria were studied with different concentrations of WT in vitro- The result showed that both comparative survival rate of budlet cells and activity of the pyruvate dehydrgenase were increased gradually with the decreases in concentration of WT- The I 50 of WT on pyruvate dehydrogenase is 29-14 μmol·L -1 - It proved that WT a...