期刊论文

中文

化学与生物工程

1672-5425

2008

25

6

38-40+45

10.3969/j.issn.1672-5425.2008.06.011

(2006BAJ02A10):国家科技部建设部科技支撑计划资助项目 (2006BAI19B05):国家科技部卫生部科技支撑计划资助项目

本校为第一机构

以戊二醛为交联剂制备了久效磷（Mcp）人工抗原（Mcp-OVA）和包被抗原（Mcp—BSA），采用紫外吸收光谱和SDS-PAGE对人工抗原进行了鉴定。取人工抗原免疫BALB／c小鼠，并采用ELISA法对小鼠抗血清效价进行了检测。结果表明，人工抗原中Mcp与OVA的偶联比为23．3：1，Mcp-OVA的最适包被浓度为1.5μg·mL^-1，3次免疫后小鼠的抗血清效价均达到1：2× 10^5以上。

Monocrotophos （Mcp） was conjugated to BSA and OVA via glutaraldehyde to make artificial antigen and coating antigen,which were identified by UV spectrometry and SDS-PAGE. BALB/c mice immunized by artificial antigen were identified by ELISA. The results showed that the conjugated ratio of Mcp to OVA in Mcp-OVA was 23.3 ： 1, the optimum coating concentration for Mcp-OVA was 1.5 μg · mL^-1. And the titers of antiserum reached above 1 ：...