期刊论文

Effect of Formaldehyde on Transcription of Cytokine M-CSF,G-CSF and EPO in the Presence and Absence of Benzene

中文

化学与生物工程

1672-5425

2016

8

31-34+38

10.3969/j.issn.1672-5425.2016.08.007

国家自然科学基金资助项目（51136002）；

本校为第一机构

研究了甲醛在有无苯作用下对3种细胞因子巨噬细胞集落刺激因子（M-CSF）、粒细胞集落刺激因子（GCSF）和促红细胞生成素（EPO）转录水平的影响。将30只雄性BALA/c小鼠随机分成5组,设为空白对照组、玉米油对照组、苯染毒组、甲醛染毒组、苯与甲醛联合染毒组,每组6只,分别进行玉米油溶液、苯溶液灌胃染毒或甲醛气态吸入染毒;染毒时间模拟工厂工作时间,每天8h,连续两周,第6d、第7d休息,第13d染毒结束;取小鼠的骨髓和肾脏,提取其总RNA,并用实时荧光定量PCR技术对细胞因子M-CSF、G-CSF和EPO转录水平的变化进行检测。结果表明,甲醛单独作用可使M-CSF、G-CSF、EPO的转录水平下降;与苯联合后,苯加强了甲...

Effects of formaldehyde on the transcription level of macrophage colony stimulating factor(M-CSF),granulocyte colony stimulating factor(G-CSF)and erythropoietin(EPO)in the presence and absence of benzene were investigated.Thirty BALA/c male mice were randomly divided into 5 groups(6 for each group):blank control group,corn oil control group,benzene exposure group,formaldehyde exposure group,benzene as-sociated with formaldehyde exposure group.And the mice were exposed by intragastric administration of corn oil solution or benzene solution,forma...