A simple, rapid and specific chemiluminescence (CL) method for the determination of glutathione has been developed. The method is based on the enhanced CL of the reaction between Ru(phen)3 2+ (phen = 1,10-phenanthroline) and KMnO4 by glutathione in HCl medium. Under the optimum conditions, the response is linearly proportional to the concentration of glutathione between 1.5 × 10-7 and 1.0 × 10-5 mol L-1. The dection limit for glutathione (5.8 × 10-8 mol L-1) is about 10 and 200 times better than those of the spectrophotometric method using E...