期刊论文

Zou, Y.

中文

分析化学

0253-3820

1998

26

2

162-165

本校为第一机构

利用双缩脲试剂，在可见光区建立了N-磷酸化二肽、二肽以及磷酸化多肽的分光光度定量分析方法。在经典的双缩脲分光光度法的实验条件下，观察到Ｎ－磷酸化二肽、二肽与双缩脲试剂的复合物的最大吸收波长均为 620 nm及 N-磷酰基对二肽有很强的增色作用。

Spectrophotometric mehtod for the determination of N-phosphoryl dipeptides and multipeptides was established by using biuret as the developer. N-phosphoryl dipeptides and dipeptides formed two colored complexes with the reagent in the media of basic cupric sulfate. It was observed that these two complexes has the same spectrum. Their maximum absorption wavelength, λmax was located at 620 nm. It was also observed that there...