期刊论文

Yuan Jun-Lin

中文

分析化学

0253-3820

2018

46

1

27-32

10.11895/j.issn.0253.3820.170206

(No.21577045)资助 This work was supported by the National Natural Science Foundation of China (21577045):国家自然科学基金项目

本校为第一且通讯机构

以化合物2-甲基-4-三氟甲基-5-噻唑甲酸(MTCA)为噻呋酰胺的半抗原合成人工抗原,采用活泼酯法合成免疫原MTCA-BSA,分别采用活泼酯法、混合酸酐法和N,N'-羰基二咪唑/4-二甲氨基吡啶(CDI / DMAP)法合成3种包被原MTCA-OVA-1、MTCA-OVA-2和MTCA-OVA-3。免疫动物后,最终以包被原MTCAOVA- 3筛选并获得了具有高特异性的噻呋酰胺多克隆抗体,建立了检测噻呋酰胺的间接竞争酶联免疫吸附(ic-ELISA)分析方法。本方法线性检测范围为0.08 ~ 10.00 mg /L,半数抑制浓度(IC_(50))为1.39 mg /L,检出限为0.08 mg /L,对自来水、湖水和小麦中的噻呋酰胺添加回收率在72.0%~ 128.3%之间,检测结果与HPLC法具有良好的相关性...

In the present study, 2-methyl-4-(trifluoromethyl) thiazole-5-carboxylic acid (MTCA) was acted as hapten of thifluzamide to synthesize artificial antigens. Immunogen MTCA. bovine serum albumin (BSA) was synthesized by active ester method. Meanwhile, three coating antigens, MTCA-OVA-1, MTCA-OVA-2 and MTCA-OVA-3 were synthesized by active ester method, mixed anhydride method and N, N- carbonyldiimidazole/4-dimethylaminopyridine (CDI/DMAP) method, respectively. Anti. thifluzamide polyclonal antibody with high specificity was obtained from the immunized mice, then indirect competitive enzyme linke...