Recent developments in high-resolution mass spectrometry (HRMS) technology enabled ultrasensitive detection of proteins, peptides, and metabolites in limited amounts of samples, even single cells. However, extraction of trace-abundance signals from complex data sets (m/z value, separation time, signal abundance) that result from ultrasensitive studies requires improved data processing algorithms. To bridge this gap, we here developed "Trace", a software framework that incorporates machine learning (ML) to automate feature selection and optimiza...