Cell-Based Ambient Venturi Autosampling and Matrix-Assisted Laser Desorption Ionization Mass Spectrometric Imaging of Products

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成果类型：

期刊论文

作者：

Shen, Baojie;Yang, Xiaoyu;Noll, Sarah Elizabeth;Yang, Xiaojie;Liu, Yanping;...

通讯作者：

Zhong, HY

作者机构：

[Shen, Baojie; Yang, Xiaojie; Zheng, Shi; Zhong, Hongying; Liu, Yanping; Yang, Xiaoyu; Zhong, HY; Jia, Shanshan] Cent China Normal Univ, Minist Educ, Lab Mass Spectrometry, Coll Chem,Key Lab Pesticides & Chem Biol, Wuhan 430079, Hubei, Peoples R China.

[Zhao, Jiaxing; Zhong, Hongying; Zhong, HY] Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop AgroBi, Nanning 530004, Guangxi, Peoples R China.

[Noll, Sarah Elizabeth; Zare, Richard N.] Stanford Univ, Dept Chem, Stanford, CA 94305 USA.

通讯机构：

[Zhong, HY ] C

Cent China Normal Univ, Minist Educ, Lab Mass Spectrometry, Coll Chem,Key Lab Pesticides & Chem Biol, Wuhan 430079, Hubei, Peoples R China.

Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop AgroBi, Nanning 530004, Guangxi, Peoples R China.

语种：

英文

期刊：

Analytical Chemistry

ISSN：

0003-2700

年：

2022

卷：

期：

页码：

3456-3466

DOI：

10.1021/acs.analchem.1c03625

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 81761128005, 21834002]; Guangxi University; Central China Normal University

机构署名：

本校为第一且通讯机构

院系归属：

化学学院

摘要：

A cell-based ambient Venturi autosampling device was established for the monitoring of dynamic cell secretions in response to chemical stimulations in real time with temporal resolution on the order of a second. Detection of secretory products of cells and screening of bioactive compounds are primarily performed on an ambient autosampling probe and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. It takes advantage of the Venturi effect in which the fluid flowing through an inlet capillary tube is automatically fed into a ...

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Cell-Based Ambient Venturi Autosampling and Matrix-Assisted Laser Desorption Ionization Mass Spectrometric Imaging of Products

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