Electrophoresis of Phosphoproteins on a Tandem Polymerized Gel

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期刊论文

作者：

Zhang, Wenyang;Leng, Xiebin;Qi, Yinghua;Yang, Xiaojie;Jiang, Ruowei;...

通讯作者：

Zhong, Hongying(hyzhong@gxu.edu.cn)

作者机构：

[Yang, Xiaojie; Leng, Xiebin; Zhong, Hongying; Qi, Yinghua; Jiang, Ruowei; Tan, Ying; Zhang, Wenyang; Yang, Xiaoyu; Hu, Xuewen] Cent China Normal Univ, Lab Mass Spectrometry, Coll Chem, Key Lab Pesticides & Chem Biol,Minist Educ, Wuhan 430079, Hubei, Peoples R China.

[Zhang, Wenyang] Guangdong Acad Agr Sci, Guangdong Key Lab Crop Germplasm Resources Preven, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China.

[Zhong, Hongying] Guangxi Univ, Ctr Instrumental Anal, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China.

通讯机构：

[Hongying Zhong] L

Laboratory of Mass Spectrometry, College of Chemistry, Central China Normal University, Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, Wuhan, Hubei 430079, P. R. China<&wdkj&>Center for Instrumental Analysis, College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi 530004, P. R. China

语种：

英文

期刊：

Analytical Chemistry

ISSN：

0003-2700

年：

2022

卷：

期：

页码：

7466-7474

DOI：

10.1021/acs.analchem.1c04404

基金类别：

National Natural Science Foundation of China [NSFC 21834002, 81761128005]; Central China Normal University; Guangxi University

机构署名：

本校为第一机构

院系归属：

化学学院

摘要：

A substrate with n phosphorylated sites may have 2n phosphor-forms for temporal-spatial regulation of biological events. Because phosphates do not significantly change molecular masses but net charges of proteins, those isoforms cannot be separated by regular mass-based sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). A tandem polymerized gel was developed to resolve phosphor-isoforms with different masses, charges, and posttranslational modifications. Without the usage of SDS, the electrophoresis was primarily performed on...

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Electrophoresis of Phosphoproteins on a Tandem Polymerized Gel

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