Nanozymes with peroxidase (POD)-like activity have garnered significant attention due to their exceptional performance in colorimetric assays. However, nanozymes often possess oxidase (OD) and POD-like activity simultaneously, which affects the accuracy and sensitivity of the detection results. To address this issue, inspired by the catalytic pocket of natural POD, a single-atom nanozyme with FeN(5) configuration is designed, exhibiting enhanced POD-like activity in comparison with a single-atom nanozyme with FeN(4) configuration. The axial N atom in FeN(5) highly mimics the amino acid residue...
