期刊论文

The Role of CodY in the Regulation of Flagellar Motility and Virulence in Listeria monocytogenes

中文

中国生物工程杂志

1671-8135

2017

37

7

56-63

10.13523/j.cb.20170711

31571931:国家自然科学基金 CCNU15A02026:中央高校基本科研业务费专项华中师范大学项目

本校为第一机构

目的:探究全局性转录调控因子CodY在单核细胞增生李斯特菌( Listeria monocytogenes, Lm)鞭毛运动和细菌毒力方面的作用。方法:通过同源重组的方法敲除Lm染色体上CodY的编码基因codY并成功构建缺失菌株的回复菌株;利用平板泳动法观测鞭毛运动的变化,RT-qPCR检测与鞭毛运动相关基因的转录表达;比较野生型菌株EGDe与CodY缺失菌株对细菌溶血活性、棉铃虫幼虫的半致死剂量和主要的毒力因子LLO和毒力基因调控蛋白PrfA转录表达的影响。结果:同野生型菌株相比,CodY缺失菌株鞭毛运动和相关基因,以及主要的毒力因子LLO和PrfA的转录表达显著降低( P≤0.01),溶血活性显著降低( P≤0.01),对棉铃虫幼虫的半致...

Objective ：To explore the function of the transcription factor CodY on flagellar motility, bacterial virulencein Listeria mortocytogenes. Methods： The codY gene on the L. monocytogenes chromosome was knocked out by homologous recombination and the strain of the deleted codY gene was successfully complemented by the codY gene construct; the results were evaluated by observation of the motilityof flagellar movement, the transcriptional expression of flagellum-related genes with RT-qPCR. The effects of wild-type strain EGDe and CodY-deficient st...