期刊论文

Effect of Glutamate Dehydrogenase Deletion on Biofilm Formation, Virulence and Extracellular Proteins Expression of Listeria monocytogenes

中文

中国生物工程杂志

1671-8135

2018

38

9

1-11

10.13523/j.cb.20180901

国家自然科学基金（31571931） 遗传调控与整合生物学湖北省重点实验室开放课题（GRIB201709） 华中师范大学中央高校基本科研业务费（CCNU18TS024）资助项目

本校为第一机构

目的:探究谷氨酸脱氢酶缺失对单核细胞增生李斯特菌(Listeria monocytogenes,Lm)生物被膜、毒力及胞外蛋白表达的影响。方法:利用微孔板法,检测谷氨酸脱氢酶缺失对Lm生物被膜形成能力的影响;比较野生株、谷氨酸脱氢酶缺失株EGDeΔgdhA及回复菌株EGDeΔgdhA + pERL3-gdhA的溶血活性和对棉铃虫幼虫的半数致死量,检测谷氨酸脱氢酶缺失对Lm毒力的影响;利用iTRAQ技术对Lm野生株EGDe与谷氨酸脱氢酶缺失株EGDeΔgdhA的胞外蛋白进行分离与鉴定,并对差异表达蛋白质进行生物信息学分析。结果:与野生株相比,缺失株形成的生物被膜量显著降低(P≤0. 01) ,溶血活性下降,对棉铃虫幼虫的半数致死量上升了1. 6倍;...

Objective: To explore the effect of glutamate dehydrogenase deletion on biofilm formation, virulence and extracellular proteins expression of Listeria monocytogenes. Methods: The effect of glutamate dehydrogenase deletion on the formation of L. monocytogenes biofilms was examined using a microtiter plate method. The hemolytic activity,the semi-lethal dose of Helicoverpa armigera (Hubner) in wild-type,GDHdeficient and revertant strains were compared. The differentially expressed extracellular proteins between the wild strain and GDH-deficient st...