期刊论文

Zhang, H.

英文

Analytical Letters

0003-2719

2007

40

4

661-676

10.1080/00032710601017730

Received 20 May 2006; accepted 5 September 2006 The authors are grateful financial support from the education department of Hubei Province (D200522003, Z200622001), China. Address correspondence to H. Zhang, Hubei Key Laboratory of Bioanalytical Technique, Department of Chemistry, Hubei Normal University, Huangshi 43500, P. R. China. E-mail: hailizh@163.com

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A novel amperometric sensor of hydrogen peroxide was constructed. Hemoglobin (Hb) was successfully immobilized on nanometer-sized SiO2, which was supported by chitosan. Chitosan was acted as dispersant. The determination of hydrogen peroxide was performed in the presence of an electron mediator hydroquinone. Hb immobilized on the SiO2/chitosan composite film displayed excellent electrocatalytical activity to the reduction of H2O2. The linear range of detection towards H2O2 was from 6.25 × 10-7 to 1.63 × 10-4mol/L with a detection limit of 1.8...