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Two isozymes of P450nor of Cylindrocarpon tonkinense: Molecular cloning of the cDNAs and genes, expressions in the yeast, and the putative NAD(P)H-binding site

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成果类型:
期刊论文
作者:
Kudo, T;Tomura, D;Liu, D L;Dai, X Q;Shoun, H
通讯作者:
Shoun, H.
作者机构:
[Tomura, D; Shoun, H; Kudo, T; Dai, X Q] Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan
Department of Biology, Huazhong Normal University, Wuhan, Hubei 430070, China
[Liu, D L] Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan, Department of Biology, Huazhong Normal University, Wuhan, Hubei 430070, China
通讯机构:
[Shoun, H.] I
Institute of Applied Biochemistry, University of Tsukuba, Japan
语种:
英文
关键词:
Cylindrocarpon tonkinense;Cytochrome P450nor;Nitric oxide reductase;P450nor
期刊:
Biochimie
ISSN:
0300-9084
年:
1996
卷:
78
期:
8-9
页码:
792-799
机构署名:
本校为其他机构
院系归属:
生命科学学院
摘要:
The cDNAs and genes for two isozymes of cytochrome P450nor of the fungus Cylindrocarpon tonkinense, P450nor1 and P450nor2, were cloned and sequenced. Their deduced amino acid sequences respectively showed 83 and 70% identity to that: of P450nor of Fusarium oxysporum, and 69% identity to each other. The genes for P450nor1 and P450nor2 were termed, respectively, CYP 55A2 and CYP 55A3. The cDNA for P450nor1 contained a targeting-like presequence upstream the N-terminus of mature protein whereas that for P450nor2 did not, suggesting their different intracellular localisations. We also succeeded in...

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