期刊论文

中文

生态毒理学报

1673-5897

2008

3

3

262-267

国家高技术研究发展规划（863）项目（No. 2006AA03Z330）；

本校为第一机构

为了探讨纳米水滑石与Hela细胞的生物相容性,采用单细胞凝胶电泳法检测了纳米水滑石对Hela细胞DNA的损伤,并采用H_2DCF-DA荧光法检测了纳米水滑石对Hela细胞活性氧簇的影响.结果发现,随着纳米水滑石浓度的升高(0～800μg·mL~(-1)),Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量均呈逐渐升高趋势;较低浓度(50、100μg·mL~(-1))纳米水滑石处理后,Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组无显著性差异(p>0.05),而较高浓度(200、400、800μg·mL~(-1))纳米水滑石处理后,Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组差异显著(p

In order to explore the biocompatibility of nano-layered double hydroxides （nano-LDHs） with Hela cells, a single cell gel electrophoresis and H2DCF-DA fluorescent coloration assay were used to analyze the DNA damage and the change of reactive oxygen species （ROS） in Hela cells exposed to nano-LDHs. Results showed that, with the increase of concentrations of nano-LDHs （0-800μg.mL^-1）, the Tail DNA%, Tail Moment and ROS contents in Hela cells increased gradually; There were significant differences （p〈0.05, p〈0.01） in Tail DNA%, Tail Mo...