期刊论文

中文

生态毒理学报

1673-5897

2008

3

5

438-442

Supported by the Grant of National High-Tech R＆D Program （863 Program） （No. 2006AA03Z330） the Grants of China National Key Technology R＆D Programs （No. 2006BAI19B05 No. 2006BAJ02A10）Acknowledgements：This work was supported by the Grant of National High-Tech R＆D Program （ 863 Program） （No. 2006AA03Z330）and the Grants of China National Key Technology R＆D Programs （No. 2006BAI19B05 No.2006BAJ02A10 ）.

本校为第一机构

为了观察二氧化锰颗粒物所致的尺度依赖性DNA损伤作用,将纳米尺度二氧化锰颗粒物（Nano-MnO2）和常规尺度二氧化锰颗粒物（Nor-MnO2）所致的DNA损伤进行了对比研究.将Hela细胞分别暴露于不同浓度（0、100、200、400μg·mL^-1）的Nano-MnO2和Nor-MnO2中,染毒24h,采用彗星实验检测Hela细胞的DNA损伤水平.结果表明：与对照组相比,Nano-MnO2和Nor-MnO2均可使彗尾DNA百分比（TailDNA%）和尾矩（TailMoment）显著增加（p〈0.01）;而在同一浓度水平上,Nano-MnO2所致的DNA损伤则比Nor-MnO2所致的DNA损伤更为严重（p〈0.01）.结果提示：二氧化锰颗粒对Hela细胞DNA损伤具有尺度依赖性毒作用,纳米尺度比常...

A comparison was made for the size-dependent DNA damage induced by both nano dioxide manganese particles（Nano-MnO_2）and normal size dioxide manganese particles（Nor-MnO_2）. Hela cells were exposed to different concentrations of both Nano-MnO_2 and Nor-MnO_2 （0, 100, 200, 400μg·mL~(-1)）, respectively. The DNA damage of Hela cells was measured by the comet assay after 24h MnO_2-cultivated. Results showed that the Tail DNA% and Tail Moment were both significantly increased after exposed to both Nano-MnO_2 and Nor-MnO_2 when compared with th...