期刊论文

中文

生态毒理学报

1673-5897

2016

11

4

124-129

10.7524/AJE.1673-5897.20160309002

国家自然科学基金重点项目

本校为第一机构

许多具有氧化作用的空气污染物,均能使细胞产生氧化损伤,使胸腺基质淋巴生成素(thymicstromallymphopoietin,TSLP)含量上升。而TSLP是一种启动过敏性炎症的重要因子,会导致哮喘等疾病发生率的上升。在本研究中用过氧化氢(H_2O_2)模拟具有氧化作用的空气污染物进行染毒,研究细胞氧化应激水平的变化,并讨论还原型谷胱甘肽(GSH)对细胞受氧化损伤的保护作用。将大鼠支气管上皮细胞(RTE)分组培养,每组设置6个平行实验,分别用低、中、高剂量H_2O_2染毒3h;高剂量设置1个重复,作为保护组,在染毒前用GSH保护2h。结果显示,高剂量组H_2O_2(3.2mmol·L~(-1))染毒的细胞,其细胞活力下降(P

It has been acknowledged that many oxidative air pollutants can cause the oxidative stress and increase the levels of thymic stromal lymphopoietin (TSLP). TSLP, a kind of important allergic disease initiator, can lead to higher incidence of diseases such as asthma. In this study, H_2O_2 was used to simulate the oxidative pollutants to stimulate cells, in order to study the changes of oxidative stress level and the protective effects of GSH. The rat tracheal epithelial (RTE) cells were cultured in six parallel test groups, including the low, med...