期刊论文

Study on Oxidative Damage of Mouse Lung Cells Induced by Plasticizers Diisononyl Phthalate

中文

生态毒理学报

1673-5897

2016

11

2

702-707

10.7524/AJE.1673-5897.20150824002

湖北省自然科学基金面上项目（2014CFB284） 国家自然青年科学基金项目（21507026） 湖北科技学院博士启动基金项目（BK1412）

本校为其他机构

为研究增塑剂邻苯二甲酸二异壬酯(diisononyl phthalate, DINP)对小鼠肺组织的氧化损伤作用,以昆明小鼠为受试动物,随机分为5组,包括1个阴性对照组(生理盐水)和4个DINP染毒组(0.2、2、20和200 mg·kg~(-1)),灌胃14 d。光镜下发现小鼠肺组织形态随染毒剂量的增加,小鼠肺细胞的病理损伤越严重。随着DINP染毒剂量的增加,肺组织匀浆活性氧(reactive oxygen species, ROS)、丙二醛(malondialdehyde, MDA)含量和肺组织细胞DNA-蛋白质交联(DNA-protein crosslink, DPC)系数逐渐上升,还原型谷胱甘肽(glutathione, GSH)含量逐渐降低,各指标呈一定的剂量-效应关系。染毒剂量为20 mg·kg~(-1)时,ROS和MDA含...

To evaluate oxidative damages of plasticizers diisononyl phthalate (DINP) on mouse lung tissue cell, Kunming mice were divided randomly into five groups and administered daily for two weeks. The groups included one saline group and four DINP groups (0.2, 2, 20 and 200 mg·kg~(-1)). Lung tissue sections were isolated and stained for pathological observations under the microscope. Meanwhile, contents of reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) in lung tissue homogenates, and DNA-protein crosslink (DPC) coefficien...