期刊论文

Effects of Different Formaldehyde Exposure Duration on Oxidative Stress and IL-17 Expression in the Pulmonary Tissue of a Mouse Asthma Model

中文

生态毒理学报

1673-5897

2017

12

1

251-259

10.7524/AJE.1673-5897.20160503002

国家自然科学基金面上项目（No:21577045）；

本校为第一机构

为了探究不同暴露时间甲醛对小鼠哮喘模型肺氧化应激及IL-17表达的影响,用浓度为3.0 mg·m~(-3)的甲醛气体吸入染毒,同时将48只雄性Balb/c小鼠随机分为6组: (1)对照组(生理盐水组); (2)ovalbumin(OVA)致敏组; (3)0.5 h甲醛+OVA组; (4)1 h甲醛+OVA组; (5)1.5 h甲醛+OVA组; (6)2 h甲醛+OVA组,以不同时间长度进行甲醛暴露,连续35 d。OVA致敏组、0.5 h甲醛+OVA组、1 h甲醛+OVA组、1.5 h甲醛+OVA组、2 h甲醛+OVA组均在第11、18及25天腹腔注射OVA致敏液(5 mg OVA+ 175 mg Al(OH)_3+30 mL生理盐水),第29~ 35天(共计1周)进行1% OVA雾化(30 min·d~(-1)),每日1次,诱发哮喘。第36天进行以下操作:取肺组织测...

In this study, an ovalbumin (OVA)-sensitized mice asthma model was established to investigate the effect of different exposure duration of 3.0 mg·m~(-3) formaldehyde (FA) in pulmonary oxidative stress and IL-17 expression. 48 male Balb/c mice were randomly divided into 6 groups: (1) saline control group, (2) OVA-sensitized group, (3) OVA-combined with 0.5 h FA group, (4) OVA-combined with 1 h FA group, (5) OVA-combined with 1.5 h FA group, (6) OVA-combined with 2 h FA group. The mouse asthma model was developed by OVA sensitization and challen...