期刊论文

Du, Dan

英文

Analytica Chimica Acta

0003-2670

2011

699

1

44-48

10.1016/j.aca.2011.05.010

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21075047]; Program for Chenguang Young Scientist for Wuhan [200950431184]; Special Fund for Basic Scientific Research of Central Colleges [CCNU10A02005]; NIH through the National Institute of Neurological Disorders and Stroke, NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Neurological Disorders & Stroke (NINDS) [U01 NS058161-01]; DOEUnited States Department of Energy (DOE) [DE-AC05-76L01830]; NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Neurological Disorders & Stroke (NINDS) [U01NS058161, U01NS058161, U01NS058161, U01NS058161, U01NS058161, U01NS058161] Funding Source: NIH RePORTER

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We reported a graphene-based immunosensor for electrochemical quantification of phosphorylated p53 on serine 15 (phospho-p5315), a potential biomarker of gamma-radiation exposure. The principle is based on sandwich immunoassay and the resulting immunocomplex is formed among phospho-p53 capture antibody, phospho-p5315 antigen, biotinylated phospho-p5315 detection antibody and horseradish peroxidase (HRP)-labeled streptavidin. The introduced HRP results in an electrocatalytic response to reduction of hydrogen peroxide in the presence of thionine....