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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1

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成果类型:
期刊论文
作者:
Klionsky, Daniel J.*;Abdel-Aziz, Amal Kamal;Abdelfatah, Sara;Abdellatif, Mahmoud;Abdoli, Asghar;...
通讯作者:
Klionsky, Daniel J.
作者机构:
[Huang, Yuxiang Jack; Hawkins, Wayne D.; Zhang, Zhihai; Lei, Yuchen; Lahiri, Vikramjit; Klionsky, Daniel J.; Ariosa, Aileen R.; Yin, Zhangyuan; Wen, Xin; Yang, Ying; Gatica, Damian; Lin, Jiandie D.; Delorme-Axford, Elizabeth; Metur, Shree Padma; Popelka, Hana] Univ Michigan, Life Sci Inst, Ann Arbor, MI 48109 USA.
[Huang, Yuxiang Jack; Hawkins, Wayne D.; Xu, Haoxing; Zhang, Zhihai; Lei, Yuchen; Zhang, Xiaoyan; Lahiri, Vikramjit; Klionsky, Daniel J.; Li, Ming; Yin, Zhangyuan; Wen, Xin; Yang, Ying; Gatica, Damian; Metur, Shree Padma; Wang, Yanzhuang] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA.
[Abdel-Aziz, Amal Kamal] Ain Shams Univ, Fac Pharm, Dept Pharmacol & Toxicol, Cairo, Egypt.
[Chiocca, Susanna; Abdel-Aziz, Amal Kamal] European Inst Oncol IRCCS, Dept Expt Oncol, IEO, Milan, Italy.
[Efferth, Thomas; Abdelfatah, Sara] Johannes Gutenberg Univ Mainz, Inst Pharmaceut & Biomed Sci, Dept Pharmaceut Biol, Mainz, Germany.
通讯机构:
[Klionsky, Daniel J.] U
Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA.
语种:
英文
关键词:
Autophagosome;cancer;flux;LC3;lysosome;macroautophagy;neurodegeneration;phagophore;stress;vacuole
期刊:
Autophagy
ISSN:
1554-8627
年:
2021
卷:
17
期:
1
页码:
1-382
基金类别:
Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BBS/E/B/000C0413] Funding Source: Medline; British Heart FoundationBritish Heart Foundation [PG/17/14/32867] Funding Source: Medline; Medical Research CouncilUK Research & Innovation (UKRI)Medical Research Council UK (MRC)European Commission [MR/M00869X/2, MR/S009426/1, MR/R009732/1, UKDRI-2002, MR/S032304/1, MR/R025096/1, G0501003, MR/N022696/1] Funding Source: Medline; NCI NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [R01 CA247992, R01 CA190370, R35 CA232113, R21 CA209345, R01 CA200310, R01 CA233794, R01 CA140964, K08 CA230151, R01 CA244144, R01 CA197398, R35 CA220446, R01 CA238457, R01 CA184137, R01 CA227838, R01 CA181196] Funding Source: Medline; NEI NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Eye Institute (NEI) [P30 EY001583, R01 EY019643, R01 EY026885, R01 EY029675, R01 EY027733] Funding Source: Medline; NHLBI NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Heart Lung & Blood Institute (NHLBI) [R01 HL132318, R01 HL133545, R01 HL142879, R01 HL072166, R01 HL085629, R01 HL118558, R01 HL107594, R01 HL123340, R01 HL154147, R01 HL088256, R01 HL153614, R01 HL153599, R01 HL141759] Funding Source: Medline; NIAAA NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Alcohol Abuse & Alcoholism (NIAAA) [P50 AA011999] Funding Source: Medline; NIAID NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [R01 AI132697, R01 AI136921, R01 AI139046, K08 AI102971, R01 AI121244] Funding Source: Medline; NIAMS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) [R01 AR061370, R01 AR077440] Funding Source: Medline; NIA NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Aging (NIA) [K08 AG050808, R01 AG062475, R01 AG061447, R01 AG062375, R01 AG049780, R56 AG063820, P01 AG054407, RF1 AG058476] Funding Source: Medline; NIDDK NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) [P30 DK020572, R01 DK107220, R01 DK112698, R01 DK093668, R01 DK121545, R01 DK124901, P01 DK098108, R01 DK124308, R01 DK099558, R01 DK117965, R01 DK113170, R01 DK108921] Funding Source: Medline; NIEHS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Environmental Health Sciences (NIEHS) [R01 ES029092, P30 ES006694, R01 ES031253] Funding Source: Medline; NIGMS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R35 GM122536, R35 GM131919, U54 GM104942, R35 GM130331, R01 GM118660, R01 GM102297, R01 GM115517, R15 GM102846, R35 GM119571, R01 GM119160, R01 GM111295, R35 GM127029] Funding Source: Medline; NINDS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Neurological Disorders & Stroke (NINDS) [R01 NS093843, R01 NS118146, P01 NS097197, R25 NS070682, R01 NS095634, R15 NS075684, R01 NS105971, R01 NS115876, R01 NS093362, R01 NS103981, R01 NS115403] Funding Source: Medline; BLRD VA [I01 BX004235, I01 BX000774, I01 BX004444, I01 BX004306, I01 BX003803] Funding Source: Medline; Pancreatic Cancer UK [FLF2015_02_BARTS] Funding Source: Medline; BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BBS/E/B/000C0413, BBS/E/F/00044500, BBS/E/B/000C0434, BBS/E/F/000PR10355, BB/R019258/1, BBS/E/T/000PR9819] Funding Source: UKRI; MRCUK Research & Innovation (UKRI)Medical Research Council UK (MRC) [MR/R025096/1, MR/S009426/1, G0501003, MR/N022696/1, MR/M00869X/2, MR/R009732/1] Funding Source: UKRI; UKRI [MR/S032304/1] Funding Source: UKRI
机构署名:
本校为其他机构
院系归属:
生命科学学院
摘要:
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we p...

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