期刊论文

RAPD Study of the Genetic Diversity of Butterflies in Houhe National Nature Reserve

中文

生物技术

1004-311X

2005

15

2

19-21

10.16519/j.cnki.1004-311x.2005.02.009

湖北省自然科学基金资助项目（2002AB106）；

本校为第一机构

目的:利用随机扩增多态性DNA(RAPD)技术对湖北省五峰后河国家级自然保护区的蝶类遗传多样性分析.方法:采用RAPD技术,对湖北省五峰后河国家级自然保护区的碧凤蝶(Papilio bianor Cramer)和曲纹蜘蛱蝶(Araschnia doirs Leech)的遗传多样性进行研究,并应用RAPDistance软件及MEGA程序,计算Nei氏相似系数和遗传距离,建立了UPGMA和NJ聚类图谱.结果:在事先优化的反应条件下用11个随机引物扩增,共得到136条片段,片段长度为170-2000bp.结论:利用相似性系数及遗传距离进行的聚类分析结果,这两种蝴蝶的不同季节种群的遗传组成存在季节变化.

Objiect:Study the genetic diversity of the butterflies in Houhe National Nature Reserve.Methods:The genetic diversity of Papilio bianor Cramer and Araschnia doris Leech populations in Houhe National Nature Reserve was investigated using the randomly amplified polymorphic DNA (RAPD) method through the polymersae chain reaction (PCR).Nei's similarity coefficients and genetic distance were calculated using the RAPDistance and MEGA softwares.The phylogenetic trees were constructed with the methods of UPGMA and NJ.Results:Amplifications with 11 primers under predetermined optimal recation condition...