期刊论文

Catalytic spectrophotometry with enzyme for determination of formaldehyde in wine

中文

食品科技

1005-9989

2006

31

1

111-113

10.3969/j.issn.1005-9989.2006.01.036

湖北省自然科学基金重点攻关项目（992P0315）.

本校为第一机构

研究了HCHO/NAD+/FDH体系的最佳条件,建立了简便测定甲醛的吸光光度法。实验发现:在温度为35℃、pH为7.0的条件下催化反应15min,所形成的催化反应产物在340nm处有最大吸收,甲醛含量在0.01452～0.2904μmol/mL范围内成良好线性关系,回归方程为:A=2.6604C-0.02476（C为μmol/mL）,其测定相关系数为0.9998,检测限达0.0100μmol/mL（S/N=3）,本法所用仪器简单、操作方便、选择性好,应用于酒中甲醛的测定结果令人满意。

The reaction conditions of HCHO/NAD~/formaldehyde dehydrogenase catalytic system were studied. The spectrophotometry method for determination of HCHO was developed. The results showed that at temperature 35℃, pHT.0, after reacting 15 minutes, the products of catalytic system exhibited maximum absorption at 340nm, the linear range for determination of HCHO was found to be 0.01452-0.2904μmol/mL with a correlation coefficient of 0.9998 and the regression equation is A=2.6604C-0.02476（C：μmol/mL）. The detection limit of this method is 0.0100μ...