期刊论文

In Vitro Whole-cell Patch Clamp Recordings of Neurons in Subnuclei of Mouse Medial Geniculate Body

中文

生命科学研究

1007-7847

2017

21

5

386-391

10.16605/j.cnki.1007-7847.2017.05.003

华中师范大学基本科研业务费青年教师项目

本校为第一机构

内侧膝状体(medial geniculate body, MGB)是听觉通路在丘脑的重要中继站,为了探究小鼠MGB亚核神经元的电生理特性,使用离体全细胞膜片钳技术记录到99个MGB神经元,其中腹侧核(ventral division of MGB,MGBv)神经元36个,背侧核(dorsal division ofMGB, MGBd)神经元34个,内侧核(medial division ofMGB, MGBm)神经元29个。结果表明,神经元对去极化电流的反应具有爆发型(bursting) (16.2%, 16/99)和紧张型(tonic) (83.8%, 83/99)两种发放模式,并且所有MGB神经元在超极化电流刺激结束后均有去极化反弹(rebound),而仅有少数神经元(17.2%, 17/99)在超极化刺激过程中有超极化激活的内向电流(hyperpolari...

Medial geniculate body （MGB） is an important thalamic relay along the auditory pathway. To inves- tigate the electrophysiological properties of MGB neurons, 99 MGB neurons were obtained by using in vitro whole-cell patch clamp recording technique, including 36 neurons from the ventral division of the MGB （MGBv）, 34 neurons from the dorsal division of the MGB （MGBd） and 29 neurons from the medial division of the MGB （MGBm）. Results showed that two firing patterns including bursting （16.2%, 16/99） and tonic （83.8%, 83/ 99） were identi...