In high-throughput screening, the immobilization of bio-target shows a significant effect on the target performance, especially the conformation. This, ultimately, has an influence on the screening quality. In present article, a series of mathematical and physical models for target immobilization were established. As shown, in the immobilization process, if the plot of C*/Q versus C* is a straight-line, the drugs obtained from in vitro screening are probably applicable to in vivo environment. Otherwise, when the linearity exists between ln Q or Q and ln C*, particularly between Q and ln C*, ad...
