Tra-1 gene was partially cloned from mermithid nematode by using RT-PCR and 3′-RACE for the first time. The 5′ truncated cDNA was 800 bp in length and encoded a putative protein of 195 amino acids, which had high homology with other members of GLI zinc-finger transcription factors in the zincfinger domain. The identities were 70% -77%. The transcript levels of tra-1 were analyzed by real-time fluorescent quantitative PCR. The results showed that the tra-1 transcript presented high levels in the female adults (P 〈 0.01 ), then dropped obser...