期刊论文

Xiao, YT;Liu, KY

英文

Applied and Environmental Microbiology

0099-2240

2022

88

7

e0250521

10.1128/aem.02505-21

This work was supported by the Central Public-interest Scientific Institution Basal Research Fund (Y2019YJ06), Key Project for Breeding Genetic Modified Organisms grant (2016ZX08012004-003), Natural Science Foundation of Hubei Province of China (No.2017CFB543) and Shenzhen Scientific and Technological Foundation (JCYJ20190813115612564). We thank Wanqiong Wen (Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences) for rearing insects; and Yuying Lin (College of Life Sciences, Central China Normal University) for assistance with cytotoxicity analysis of Hi5 cells.

本校为通讯机构

Bacillus thuringiensis Cry proteins are used worldwide for insect control. It was proposed that Cry-protoxins must be converted into activated toxin by proteases to bind midgut cell proteins to kill insects. However, Cry-protoxins also bind to midgut proteins and kill insects that have evolved resistance to activated toxins suggesting an independent toxicity pathway. Cadherin (CAD) and ABCC transporters are recognized as important receptors for Cry proteins. Here we constructed different Helicoverpa armigera mutations in these receptors by CRIS...