期刊论文

Xu, Pingyong;Betzig, Eric

英文

Proceedings of the National Academy of Sciences of the United States of America

0027-8424

2016

113

37

10364-10369

10.1073/pnas.1611038113

We thank White Helen at the Janelia Research Campus for specimen preparation and X. Yu and Y. Teng at the Institute of Biophysics for providing technical support of analytical ultracentrifugation and confocal imaging, respectively. This project was supported by National Basic Research Program Grant 2013CB910103; National Key Research and Development Projects Grant 2016YFA0501500; National Natural Science Foundation of China Grants 31421002, 31370851, 31170818, and 31300612; Project of the Chinese Academy of Sciences Grant XDB08030202; Youth Innovation Promotion Association CAS Grants 2012080 and 2013067; and Beijing Natural Science Foundation Grant 7131011.

本校为其他机构

Two long-standing problems for superresolution (SR) fluorescence microscopy are high illumination intensity and long acquisition time, which significantly hamper its application for live-cell imaging. Reversibly photoswitchable fluorescent proteins (RSFPs) have made it possible to dramatically lower the illumination intensities in saturated depletion-based SR techniques, such as saturated depletion nonlinear structured illumination microscopy (NL-SIM) and reversible saturable optical fluorescence transition microscopy. The characteristics of RSFPs most critical for SR live-cell imaging include...