期刊论文

中文

植物保护学报

0577-7518

1996

23

4

343-348

湖北省重点科技攻关项目；

本校为第一机构

MTT方法是Mosmann（1983）首创的一种鉴别、统计细胞死或活的方法。本文通过改进MTT方法,以棉铃虫的细胞系（BCIRL—HA—AMI）为测试对象,建立起一种适应农药毒力测定的细胞生物测定方法。该方法比传统活体生物测定要快捷、简便、准确、灵敏。本文用改进的方法研究了5种有机溶剂和9种杀虫剂（或化合物）的离体毒力,5种有机溶剂除二甲苯外,丙酮、乙酸乙酯、二甲亚砜、N,N—二甲基甲酰胺在低浓度（0.5％,v/v）时与空白对照无显著差异。结果还表明,除溴氰菊酯和氯丹外,其它7种有机磷和氨基甲酸酯类杀虫剂（或化合物）的细胞生物测定结果与传统活体生物测定结果相关性很好,这表明MTT细胞生物测定方法...

An insect cell bioassay for pesticide was developed with an established cell line (BCIRL -HA -AMI) of Helicoverpa armigera (Hubner) (Ha) by modified MTT method. This bioassay substantially reduce the time,material,experiment error,and improved the sensitivity. It was used to study the effects of 5 organic solvents on Ha cell growth,and to evaluate the cytotoxicity of 9 insecticides (or compounds). Acetone,ethyl acetate,dimethyl sulfoxide and dimethyl formamide showed no effects on the cell growth at low concentration (0. 5% , v/v) ,except xylen...