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Modified protocol of comet assay on human buccal cell

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成果类型:
期刊论文、会议论文
作者:
Hu, JC;Yao, D;Li, R*;Lu, ZS;Qi, QP;...
通讯作者:
Li, R
作者机构:
[Yao, D; Lu, ZS; Hu, JC; Yang, X; Li, R; Qi, QP] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构:
[Li, R] C
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
语种:
英文
关键词:
comet assay;modified protocol;buccal cell;enzyme-incubation
期刊:
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5
ISSN:
0908-5920
年:
2005
卷:
15
页码:
3568-3570
会议名称:
10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
会议时间:
SEP 04-09, 2005
会议地点:
Beijing, PEOPLES R CHINA
会议主办单位:
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议赞助商:
Tsinghua Univ, Minist Educ China, Natl Nat Sci Fdn China, Beijing Municipal Commiss Sci & Technol, Shanghai Municipal Commiss Sci & Technol, US EPA, Amer Ind Hyg Assoc, Amer Soc Heating, Refrigerating & Air Conditioning Engineers, Int Commiss Occupat Hlth, SC Indoor Air Qual & Hlth, Int Council Res & Innovat Bldg & Construct, European Commiss Asia Pro Eco Programme Promot Sustainable Solut Environm Europe & Asia, Korean Soc Indoor Environm, Soc Indoor Environm
主编:
Yang, X Zhao, B Zhao, R
出版地:
TSINGHUA UNIV HAIDIANQU, BEIJING 100084, PEOPLES R CHINA
出版者:
TSINGHUA UNIV PRESS
ISBN:
978-7-89494-830-4
机构署名:
本校为第一且通讯机构
院系归属:
生命科学学院
摘要:
In order to improve the efficiency of comet assay, which can be used in epidemiological research to detect genotoxicity of air pollutants, we undertook this study. Formaldehyde at 50 mu M was employed as contaminating agent to poison the human buccal cell before comet assay. During the process of comet assay, each pair of contaminated samples was treated with proteinase K of three different concentrations at 0.1mg/ml, 1mg/ml and 10mg/ml, respectively while other steps are kept the same. The result turned out to be that the 1mg/ml proteinase K could lead to an ideal incubating effect. It was al...

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