In order to improve the efficiency of comet assay, which can be used in epidemiological research to detect genotoxicity of air pollutants, we undertook this study. Formaldehyde at 50μM was employed as contaminating agent to poison the human buccal cell before comet assay. During the process of comet assay, each pair of contaminated samples was treated with proteinase K of three different concentrations at 0.1mg/ml, 1mg/ml and 10mg/ml, respectively while other steps are kept the same. The result turned out to be that the 1 mg/ml proteinase K co...
