期刊论文

Effects of copper on rhizobia-Acacia auriculaeformis symbiotic association

中文

应用生态学报

Chinese Journal of Applied Ecology

1001-9332

2002

13

2

137-140

国家自然科学基金资助项目 ( 39870 14 5 )；

本校为其他机构

报道了两种根瘤菌(大叶相思、美丽胡枝子)对Cu2+的耐受性以及植物-根瘤菌共生固氮体系在Cu2+胁迫下结瘤、固氮和生长的变化,讨论了大叶相思在矿山尾矿废弃地作为先锋植物结瘤固氮的可能性.结果表明,大叶相思根瘤菌对Cu2+离子的耐受性较强,可以耐受Cu2+＜0.80mmol的离子浓度,Cu2+对它的半致死浓度为0.129mmol.在无菌砂培无重金属影响条件下,其固氮酶活性为2.7 C2H4*μg*g-1*h-1,当Cu2+>0.125mmol会导致大叶相思固氮酶活性急剧下降,其有效半抑制浓度(EC50)为0.151mmol,Cu2+为0.50mmol完色抑制大叶相思固氮酶活性,不阻碍结瘤,但严重抑制植物生长发育,引起植物叶片白化、植株矮化.在外加N源不接菌和...

The effects of different levels of Cu\+\{2+\} were studied individually on the growth of two Rhizobium strains and host plant growth,nodulation(%) and nitrogen fixation activity in A.auriculaeformis under solution culture conditions so as to explore a probability of utilization of the genus Acacia in revegetation of Cu\+\{2+\}-contaminated land.The results shows that Rhizobium sp.(Acacia auriculaeformis)had more stronger tolerance to Cu\+\{2+\} iron than Rhizobium sp(Lespedeza formosa).The values of EC\-\{50\} respectively achieved 0\^129mmol for Rhizobium sp.and 0\^151mmol for the N\-2-fixati...