期刊论文

Hu, S.

中文

中国组织工程研究

2095-4344

2007

11

29

5709-5711

10.3321/j.issn:1673-8225.2007.29.026

本校为其他机构

目的:分析运动强度与端粒酶表达及心肌细胞受损的关系.方法:实验于2005-04/11在华中师范大学体育学院人体科学实验室完成.①雄性SD大鼠30只,随机分为3组,对照组,有氧训练组,过度训练组,每组10只.②对照组不加任何训练;有氧训练组大鼠不负重,每天游泳训练75 min,每周训练5 d,休息2 d.过度训练组大鼠每天游泳训练180 min,每周训练5 d,休息2 d.从第4周开始,大鼠尾部负重,重物为体质量的5%,第一天负重5 min,以后每次增加10 min,直至180 min全程负重.总训练时间16周.16周后处死,于左心室处取材,常规脱水石蜡包埋,切片,行苏木精一伊红染色.端粒酶TERT mRNA的检测采用原位杂交法,TERT mRNA表达阳性被染...

Aim: To analyze the relationship of exercise intensity with telomerase expressions and cardiac myocyte damage. Methods: The experiment was carried out in the Laboratory of Sports and Human Science, Department of Physical Education, Huazhong Normal University from April to November 2005. 1 Thirty male SD rats were randomly divided into three groups including control group, aerobic exercise group, and overload exercise group with 10 rats in each group. 2 The control group did not do any exercise; the aerobic exercise group swam for 75 minutes eve...