With the application of Web 2.0 technologies, the E-Learning world has great changes. In higher education, knowledge management presents new features under Web 2.0. Based on the theoretical background of Web 2.0 and knowledge management, this paper analyzes the framework for knowledge work and constructs the knowledge management model under Web 2.0 in higher education. We carry out the learning practice research under this model and collect feedback from teachers and students. Through the effectiveness analysis, this paper gives us the advantages and something we need attention towards the new...