An optimized fluorescent biosensor for monitoring long-chain fatty acyl-CoAs metabolism in vivo

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成果类型：

期刊论文

作者：

Wang, Weibo;Wang, Pengcheng;Zhu, Lixin;Liu, Bingjie;Wei, Qingpeng;...

通讯作者：

Wang, J;Yang, GF

作者机构：

[Hou, Yongkang; Wang, Weibo; Wang, Yuan; Wang, Jing; Li, Xi; Liu, Bingjie; Zhu, Lixin; Wei, Qingpeng; Li, Wenzhe; Wang, J; Hu, Yufei] Peking Univ, Sch Pharmaceut Sci, Dept Chem Biol, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China.

[Yang, Guangfu; Yang, GF; Wang, Weibo] Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.

[Jiang, Changtao] Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, State Key Lab Vasc Homeostasis & Remodeling, Beijing 100191, Peoples R China.

[Wang, Pengcheng] Peking Univ, Hosp 3, Inst Med Innovat & Res, Ctr Basic Med Res, Beijing 100191, Peoples R China.

通讯机构：

[Yang, GF ] C

[Wang, J ] P

Peking Univ, Sch Pharmaceut Sci, Dept Chem Biol, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China.

Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.

语种：

英文

关键词：

Biosensor;Liver tissue imaging;Long-chain fatty acyl-CoA;Metabolism;Protein engineering

期刊：

Biosensors and Bioelectronics

ISSN：

0956-5663

年：

2024

卷：

247

页码：

115935

DOI：

10.1016/j.bios.2023.115935

基金类别：

National Key Research and Development Program of China [2022YFC3400600]; National Natural Science Foundation of China [22277001, 91853107]; Youth Thousand-Talents Program of China

机构署名：

本校为通讯机构

摘要：

Long-chain fatty acyl-CoAs (LCACoAs) are intermediates in lipid metabolism that exert a wide range of cellular functions. However, our knowledge about the subcellular distribution and regulatory impacts of LCACoAs is limited by a lack of methods for detecting LCACoAs in living cells and tissues. Here, we report our development of LACSerHR, a genetically encoded fluorescent biosensor that enables precise measurement of subtle fluctuations in the levels of endogenous LCACoAs in vivo. LACSerHR significantly improve the fluorescent brightness and analyte affinity, in vitro and in vivo testing show...

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An optimized fluorescent biosensor for monitoring long-chain fatty acyl-CoAs metabolism in vivo

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