期刊论文

Ma, Ping

中文

中国环境科学

1000-6923

2015

35

1

285-290

湖北省大学生创新训练计划项目（201310927010）； 湖北省自然科学基金项目（2014CFB284）； 国家自然科学基金重点项目（51136002）；

本校为其他机构

研究邻苯二甲酸二异壬酯对小鼠肝细胞的氧化损伤.以昆明小鼠为受试动物,随机分为5组,包括1个阴性对照组、4个邻苯二甲酸二异壬酯染毒组,染毒组按0.5,5,50,500mg/kg4个剂量水平,灌胃染毒小鼠14d.以肝组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)和8-羟基脱氧鸟苷(8-OHdG)的含量;以肝组织细胞测定DNA-蛋白质交联(DPC)系数.随着邻苯二甲酸二异壬酯染毒剂量的升高,肝组织的ROS、MDA、8-OHdG含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量为50mg/kg 时,ROS、GSH、8-OHdG含量和DPC系数差异有统计学意义(P< 0.05, P< 0.01);染毒剂量为500mg/kg时,上述指标...

To investigate the oxidative damages of diisononyl phthalate on mouse liver cells, Kunming mice were randomly grouped into five groups and orally administered with drugs daily for fourteen days;the groups included one solvent control group, four diisononyl phthalate groups. The exposure doses of diisononyl phthalate groups were 0.5, 5, 50 and 500 mg/kg respectively. Some liver tissues were then made into homogenates for the measurement of ROS (reactive oxygen species), GSH (glutathione), 8-hydroxy-2-deoxyguanosine (8-OHdG) and MDA (Malondialdehyde) contents. Meanwhile, DPC (DNA-protein Crossli...