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PCR-DGGE研究青海农村户用沼气池微生物群落结构

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成果类型:
期刊论文
作者:
韩睿;陈来生;李莉;刘德立
通讯作者:
Liu, De-Li
作者机构:
[刘德立; 韩睿] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan, China
[陈来生; 李莉; 韩睿] Qinghai Key Laboratory of Vegetable Genetics and Physiology, Qinghai Academy of Agriculture and Forestry Science, Xining, China
[陈来生; 韩睿] State Key Laboratory Breeding Base, Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, Xining, China
通讯机构:
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan, China
语种:
中文
关键词:
青海;户用沼气池;微生物群落结构
关键词(英文):
PCR-DGGE
期刊:
中国环境科学
ISSN:
1000-6923
年:
2015
卷:
35
期:
6
页码:
1794-1804
基金类别:
青海省蔬菜遗传与生理重点实验室(2014-Z-Y15);
机构署名:
本校为第一且通讯机构
院系归属:
生命科学学院
摘要:
采用变性梯度凝胶电泳(DGGE)分析技术,对青海农村户用沼气池内细菌及古菌群落结构进行分析.结果表明,沼气池含有丰富的细菌及古菌类群,且泥样间的细菌和古菌的群落结构存在差异.4个户用沼气池发酵系统中,细菌分属于5个门,优势类群为拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和变形菌门(Proteobacteria).在属分类水平上,属于24个类群,最优势类群为理研菌科佩特里单胞菌属(Petrimonas)、假单胞菌属(Pseudomona)、泰氏菌属(Tissierella)和梭菌属(Clostridium).同时,沼气池发酵系统中古菌主要包括热丝菌属(Thermofilum)、甲烷短杆菌属(Methanobrevibacter)、甲烷囊菌属(Methanoculleus)、产甲...
摘要(英文):
The microbial community composition in rural household biogas digesters of Qinghai was investigated with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technology. The results showed that the diversity of bacteria and archaea were rich, and the shift of microbial community was obvious in digesters. The bacterial communities in four biogas digesters were classified into five phyla, Bacteroidetes, Firmicutes and Proteobacteria were identified to be dominant. At genome level, belonging to twenty-four communities, Petrimonas, Pseudomona, Tissierella and Clostridium we...

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