期刊论文

Formaldehyde and PM_(2.5) induced hepatotoxicity in mice

Li, Rui(ruili@mail.ccnu.edu.cn)

中文

中国环境科学

1000-6923

2017

37

7

2740-2748

国家自然科学基金面上资助项目（21577045）;国家自然科学基金重点资助项目（51136002）； 中央高校基本科研业务费探索创新项目（CCNU15A02028）；

本校为第一且通讯机构

为探究甲醛(FA)复合PM_(2.5)对小鼠血液毒性的影响及其可能的机制,分别从血液,造血器官(骨髓、脾脏),髓系祖细胞3个层面的损伤进行系统研究.以雄性Balb/c小鼠为实验对象,把小鼠随机分为4组(对照组、PM_(2.5)组、FA组、PM_(2.5)+ FA组),对小鼠进行全血细胞计数;观察骨髓、脾脏病理学的变化;检测骨髓、脾脏及髓系祖细胞的氧化损伤(ROS,GSH),DNA损伤(DPC,8-OH-dG),细胞凋亡(caspase-3)指标.结果表明,骨髓、脾脏分别出现不同程度的病理学变化;骨髓、脾脏,髓系祖细胞的氧化损伤,DNA损伤,细胞凋亡水平也有上升趋势.FA复合PM_(2.5)暴露会导致小鼠的血液毒性,氧化应激,及其下游的DNA损伤可能是FA复合PM_...

In order to explore the influence and the underlying mechanism of FA and PM2.5on hepatotoxicity, we conducted systematic research from three aspects, including toxicity of blood, hematopoietic organs (bone marrow, spleen) and myeloid progenitor. Male Blab/c mice were taken as experimental object, and were randomly divided into four groups: the control group;PM2.5-exposure group;FA-exposure group;PM2.5+ FA-exposure group. Blood samples were firstly collected from mice caudal vein to conduct the complete blood count. Then the histopathological change of bone marrow and spleen (H &E staining)...