期刊论文

中文

微生物学通报

0253-2654

2017

44

1

161-171

10.13344/j.microbiol.china.160121

(No.31571931):National Natural Science Foundation of China (No.GRIB201404):The Project of Hubei Key Laboratory of Genetic Regulation and Integrative Biology (No.CCNU15A02026)国家自然科学基金项目(No.31571931):Self-determined Research Funds of CCNU from the Colleges' basic Research and Operation of MOE (No.GRIB201404):遗传调控与整合生物学湖北省重点实验室开放课题项目 (No.CCNU15A02026):华中师范大学中央高校基本科研业务费项目

本校为第一机构

【目的】探究单核细胞增生李斯特菌(Listeria monocytogenes,Lm) rmlB基因在细菌耐药、生物被膜形成和毒力方面的作用。【方法】通过同源重组的方法敲除Lm染色体上的rmlB基因,比较野生株与rmlB缺失株在耐药性方面的差异;利用微孔板法观测rmlB缺失菌株生物被膜形成能力的变化;利用RT-PCR检测缺失菌株中主要毒力基因转录表达,并观察rmlB缺失对细菌溶血活性的影响。【结果】同野生菌株相比,rmlB缺失菌株对头孢菌素和杆菌肽等作用位点在细菌细胞壁和细胞膜的敏感性显著增加(P≤0.01),生物被膜形成能力显著降低(P≤0.01),细菌主要毒力基因hly的转录表达及溶血活性也发生显著降低(P≤0.01)。【结论】 ...

[Objective] To explore the function of rmlB gene on antibiotic resistance, biofilm formation, and virulence in Listeria monocytogenes. [Methods] The rmlB gene was deleted through homologous recombination. Antibiotic resistancedifferences between the wild-type and the rmlB deletion mutant were compared. The biofilm formation ability of the mutant was measured using the microtiter plate assay. The transcription of major virulence genes in L. monocytogenes and hemolytic activity of the mutant were studied. [Results] Compared tothe wild-type strain...