期刊论文

Isolation and activity of Listeria monocytogenes-derived membrane vesicles

中文

微生物学通报

0253-2654

2021

48

01

145-155

10.13344/j.microbiol.china.200436

国家自然科学基金（31571931）； 遗传调控与整合生物学湖北省重点实验室开放课题（GRIB201709）； 中央高校基本科研业务费专项基金（CCNU18TS024）～～；

本校为第一机构

【背景】细胞外囊泡（Extracellular Vesicles,EVs）是一种在自然界中普遍存在的包含生物学活性物质的囊泡状结构,其中包括革兰氏阳性菌分泌的膜囊泡（Membrane Vesicles,MVs）。近年来,单核细胞增生李斯特菌（Listeria monocytogenes,Lm）作为一种能产MVs的革兰氏阳性致病菌引起了众多科研人员的关注,尽管如此,对其MVs的了解仍然有限。【目的】建立健全Lm-MVs制备方法,同时深入了解其生物活性。【方法】以Lm野生菌株EGDe及其毒力突变株（EGDe?prfA、EGDe?prfA+pERL3-prfA*）为研究对象,利用超滤浓缩法和Optiprep密度梯度离心法提取Lm的MVs,并对这两种提取MVs的方法进行比较。此外,通过检测不同...

[Background] Extracellular vesicles are lipid bound vesicles harboring specific subsets of bioactive proteins, lipids, nucleic acids, and metabolites, secreted by all living cells into the extracellular space. They have been named as membrane vesicles (MVs) in Gram-positive bacteria. Recently, vesicle production is described in Listeria monocytogenes, a Gram-positive intracellular foodborne pathogen that causes listeriosis in human as well as in animals. However, the knowledge about the impact of L. monocytogenes-derived MVs on host health and ...