Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect withcadherin on cytotoxicity of activated Cry1Ac
This work was supported by the National Natural Science Foundation of China ( 31071739 and 31372260 ). We thanked Dr. Lihua Huang for his advice. Prof. Yi Li, Mr. Zhongchao Gai and Mr. Haihao Ma also helped us analyze the data. Appendix A
机构署名：
本校为第一且通讯机构
院系归属：
生命科学学院
摘要：
Although many insect cell lines derived from various tissues are available, it is unclear whether endogenous receptors of Bacillus thuringiensis (Bt) crystal toxins are expressed in these cell lines. In the present study, we demonstrated that the ovaries-derived Spodoptera litura Sl-HP cell line was susceptible to activated Cry1Ac although larvae of S.litura are not susceptible to the toxin. Assays of the transcriptome revealed that thirteen ATP-binding cassette transporter genes (ABC) were expressed at different levels in this cell line. Of th...
