期刊论文

Study on Tissue Culture of Osmunda Japonica Thunb.

中文

闽南师范大学学报(自然科学版)

2095-7122

2008

21

4

108-112

10.3969/j.issn.1008-7826.2008.04.025

湖北省教育厅创新项目（2002D08001）；

本校为其他机构

紫萁孢子叶小叶整体表面消毒比孢子粉消毒更简便易行,效果也更理想.不加任何激素与附加低浓度激素(低于1.0 mg/t,)同样有很高的孢子萌发率,将MS培养基中无机盐浓度减半促进孢子萌发,糖浓度在2%-3%范围内较合适,低于2%和高出4%,孢子萌发率明显降低.25℃左右是原叶体增值的适宜温度,无机盐浓度对原叶体增殖无明显影响,培养基中附加少量活性炭和KH2PO4对原叶体增殖和生长有促进作用.6-BA0.5mg/L+NAA0.5mg/L、6-BA1.0mg/L+NAA0.5 mg/L、NAA0.5mg/L+KT 5.0mg/L和NAA1.0mg/L+KT5.0mg/L都能促进原叶体增殖.6-BA 0.5mg/L+NAA1.0mg/L分化假根的能力最强,可作孢子体生根培养基.

There was better effect on surface disinfection by using sporophyllary leaf than by using spores,and there was high germination rate of spores while the concentration of hormones were lower than 1.0 mg/L. To half the concentration of inorganic salt from MS medium was good for spores germination. The concentration of sugar was suitable from 2% to 3% or the spores germination rate would be very low. The temperature of 25℃ was suitable for prothallium proliferation. The concentration of inorganic salt could not affect obviously the prothallium pr...