In this study, a new resin semithin section staining method suitable to plant tissue-section was established. Young roots, young stems, leaves and ovules of cotton (Gossypium hirsutum) and stems of maize (Zea mays) were analyzed by the methods of resin semithin section. The improved staining methods (including safranin-fast green staining, hematoxylin-eosin staining, FeCl3-haematoxylin staining) were applied in the preparation of the plant semithin section. Our experimental results indicated that clear-staining and integral-structural semithin section of cotton and maize tissues were obtained ...