期刊论文

Study on DNA-Protein Crosslinks of Mice Liver Induced by Di - （2 - ethylhexyl） Phthalate

中文

医学研究杂志

1673-548X

2006

35

11

13-16

10.3969/j.issn.1673-548X.2006.11.006

国家自然基金面上项目（No.30570799）；

本校为第一机构

邻苯二甲酸酯是一类广泛应用于人类生产和生活的酸酞酯类人造化学物,许多研究表明其具有广泛的生物学毒性.DNA-蛋白质交联(DNA-protein crosslinks,DPC)是DNA分子的一种严重损伤.本文以小鼠肝为实验材料,采用Kcl-SDS沉淀法检测邻苯二甲酸二异辛酯(DEHP)腹腔注射染毒两周后,检测其导致的小鼠肝DPC效应.实验结果表明,低浓度的DEHP(62.5mg/kg)不能致DPC效应(P＞0.05),随着染毒浓度的倍增(125 mg/kg,250 mg/kg,375 mg/kg),其DPC系数也随之升高,可导致明显的DPC效应(P＜0.01),而在500 mg/kg染毒组,由于其染毒剂量达到小鼠的致死水平,其DPC效应虽然与空白对照组相比有极显著性差异(P＜0.01),但＜125 m...

Phthalates are a family of manufactured chemicals that are widely used in our daily life. Many researches haveindicated that it has extensive toxicities. DNA - protein crosslinks （DPC） is a kind of serious DNA damage. To study the effect of di - ethylhexyl phthalate （DEHP） induced DPC in mice liver. This study took mice liver as experimental material and made use of KCl - SDS assay to determine the amount of DPC induced by DEHP with celiac injection in vivo for two weeks. From the KCl - SDS assay results DEHP can not cause DPC at low levels...