An extra copy of p35 (p35 ORF and p35 EPO ) gene was introduced into the genome of TnNPV in order to show their effects on very late gene expression. The p35 ORF and p35 EPO fragments were oriented directly under the control of the synthetic and XIV promoters in the transfer vector pSXIVVI +X3. Two strategies were followed to construct the p35 directly oriented recombinant TnNPVs (TnNPV Δp10/LacZ + ΔHBVS/polh ++p35 ORF and TnNPV Δp10/LacZ +-ΔHBV S/polh ++p35 EPO )either by inserting lacZ gene first into the viral genome or by inserting dire...
