期刊论文

Qi, Chao

英文

Biotechnology Letters

0141-5492

2015

37

5

1049-1055

10.1007/s10529-015-1766-8

We are thankful for the kindness of Dr. Deng Hai in the university of Aberdeen (UK) to offer Salinispora arenicola CNS-205. We also thank for the support of Hubei province natural science foundation (No. 2010CDB01206 to QC) and National Natural Science foundation of China (No. 31170050 to AL and No. 20772040) and financially supported by self-determined research funds of Central China Normal University (CCNU) from the colleges’ basic research and operation of MOE (No. CCNU11A02013).

本校为第一且通讯机构

Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ‘‘amino acid adenylation domain’’located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specifically recognized and activated tryptophan (Trp) and phenylalanine (Phe). The basic kinetic parameters of Sare0357 for Trp were K<inf>m</inf> = 0.04 mM, V<inf>max</inf> = 2.1 μM/min, k<inf>cat</inf...