A sensitive and stable amperometric sensor has been devised for rapid determination of triazophos based on efficient immobilization of acetylcholinesterase (AChE) on silica sol-gel (SiSG) film assembling multiwall carbon nanotubes (MWNTs). The sol-gel matrix provided a biocompatible microenvironment around the enzyme and efficiently prevented leakage of the enzyme from the film. In the presence of acetylthiocholine chloride (ATCl) as a substrate, MWNTs promoted electron transfer reactions at a lower potential and catalyzed electrochemical oxidation of enzymatically formed thiocholine, thus inc...
